RF-O2熒光光纖氧氣測(cè)量技術(shù)是基于REDFLASH光極傳感器技的氧氣測(cè)量技術(shù)，由歐洲Pyroscience公司及Graz大學(xué)等科學(xué)家研制生產(chǎn)，由光極氧氣傳感器、測(cè)量?jī)x及軟件組成，廣泛應(yīng)用于環(huán)境科學(xué)、生態(tài)科學(xué)、植物科學(xué)、動(dòng)物科學(xué)、海洋科學(xué)、生物醫(yī)學(xué)、生物技術(shù)、食品科學(xué)等各個(gè)領(lǐng)域，其主要功能特點(diǎn)如下

1) REDFLASH光極氧氣傳感器技術(shù)，高精確度、高穩(wěn)定性、高時(shí)空解析度、低能耗、無(wú)耗氧、無(wú)交叉敏感性

2) 傳感器類型靈活多樣，有探頭式、探針式、非接觸式（sensor spot）及納米微粒式等，適應(yīng)于液體和氣體不同條件下的O2測(cè)量

3) 有內(nèi)置sensor spot的流通管和呼吸瓶，非接觸式測(cè)量流動(dòng)液體的溶解氧及呼吸瓶?jī)?nèi)液體或氣體中氧氣含量

4) 輕便緊湊型FireStingO2測(cè)量?jī)x，內(nèi)置水汽、氣壓傳感器，有1、2、4通道供選配，可分別接1個(gè)、2個(gè)、4個(gè)光極氧氣傳感器，另有Mini型FireStingO2-mini供選配

5) U盤式PiccolO2測(cè)量?jī)x——世界上小的O2測(cè)量?jī)x，可連接一個(gè)O2傳感器，USB口連接電腦，即插即用

測(cè)量原理：

REDFLASH光極O2傳感器技術(shù)，利用*的O2敏感REDFLASH指示劑，通過(guò)610-630nm調(diào)制紅光激發(fā)，REDFLASH指示劑發(fā)出760-790nm紅外熒光，熒光強(qiáng)度隨接觸的O2分子濃度升高而發(fā)生熒光淬滅，這種熒光動(dòng)態(tài)通過(guò)光纖傳輸?shù)綔y(cè)量?jī)x，測(cè)量?jī)x靈敏地檢測(cè)其相位漂移并據(jù)此換算成O2濃度。

應(yīng)用領(lǐng)域：

1) 水體溶解氧測(cè)量監(jiān)測(cè)、藻類及藻類生物膜光合作用與呼吸作用測(cè)量監(jiān)測(cè)

2) 植物光合作用與呼吸作用測(cè)量監(jiān)測(cè)

3) 水生動(dòng)物（魚類、水生昆蟲等無(wú)脊椎動(dòng)物、浮游動(dòng)物等呼吸代謝測(cè)量

4) 陸生動(dòng)物、實(shí)驗(yàn)動(dòng)物、動(dòng)物組織、血液等呼吸代謝測(cè)量

5) 土壤、濕地、海洋沉積、河湖沉積剖面O2測(cè)量

6) 生物反應(yīng)器、發(fā)酵過(guò)程、酶動(dòng)力學(xué)、細(xì)胞培養(yǎng)等O2測(cè)量監(jiān)測(cè)

7) 糧食食品儲(chǔ)運(yùn)、葡萄酒等O2測(cè)量監(jiān)測(cè)

8) 污水處理、沼氣、垃圾填埋場(chǎng)、有機(jī)物降解等O2測(cè)量監(jiān)測(cè)

技術(shù)指標(biāo)：

1) FireStingO2（FSO2）測(cè)量?jī)x：

a) 有1通道、2通道、4通道可供選配，分別可接1個(gè)、2個(gè)和4個(gè)O2傳感器，可并聯(lián)組成8通道甚至更多通道；另具備一個(gè)溫度傳感器通道（可選配4通道溫度傳感器）b) 激發(fā)光源620nm，監(jiān)測(cè)器760nm（NIR）

c) 采樣頻率：每秒4次

d) 內(nèi)置氣壓傳感器，300－1100mbar，0.06mbar分辨率，精確度±3mbar

e) 內(nèi)置濕度傳感器，0－*，分辨率0.04%，精確度±0.2%

f) 內(nèi)置溫度傳感器，-40－125°C，分辨率0.01°C，精確度±0.3°C

g) 具模擬輸出和自動(dòng)模式，0－2.5VDC

h) USB接口，通過(guò)USB口PC供電

i) 大?。?/span>68x120x30mm，重350g

2) PiccolO2 U盤式測(cè)量?jī)x：大小僅15x15x54mm，重量約20g，單通道，激發(fā)光620nm，檢測(cè)器760nm，采樣頻率每秒20次?？刹⒙?lián)組成多通道測(cè)量系統(tǒng)?？赏ㄟ^(guò)PiccoTHP測(cè)量溫濕度和氣壓并進(jìn)行補(bǔ)償

3) 探頭式O2傳感器：直徑3mm，測(cè)量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測(cè)極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，低使用壽命1千萬(wàn)數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

4) 探針式O2傳感器：有固定探針式、可伸縮探針式、尖頭式及圓頭式等不同類型供選配；探針直徑有50μm、230μm、430μm等規(guī)格；測(cè)量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測(cè)極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，響應(yīng)時(shí)間小于1s（與探針粗細(xì)有關(guān)），低使用壽命1百萬(wàn)數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

5) 非接觸式（sensor spot）O2傳感器（見下左圖）：用于非接觸性測(cè)量監(jiān)測(cè)透明容器中的氧氣含量，傳感器貼用硅膠等貼附在容器內(nèi)壁，通過(guò)固定在外壁的光纖將熒光動(dòng)態(tài)信號(hào)傳輸?shù)綔y(cè)量?jī)x以檢測(cè)O2濃度；測(cè)量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測(cè)極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，低使用壽命2千萬(wàn)數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

6) 納米微粒傳感器（參見上右圖）：納米技術(shù)，用于非接觸性測(cè)量微量液體中O2含量，即時(shí)響應(yīng)，測(cè)量范圍0-50%（0-23mg/l），檢測(cè)極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

7) 流通管：內(nèi)置非接觸式O2傳感器，用于流動(dòng)液體O2測(cè)量監(jiān)測(cè)（如魚類呼吸代謝測(cè)量等），測(cè)量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測(cè)極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，低使用壽命1千萬(wàn)數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

8) 呼吸瓶：內(nèi)置非接觸式O2傳感器，用于生物呼吸測(cè)量（如藻類、小型魚類、魚卵、昆蟲等），標(biāo)準(zhǔn)配置有4ml和20ml兩種規(guī)格

9) Pyro Oxygen Logger軟件用于參數(shù)設(shè)置、校準(zhǔn)、數(shù)據(jù)顯示包括圖表顯示、數(shù)據(jù)輸出等功能

應(yīng)用案例：

案例1：法國(guó)Bordeaux大學(xué)利用FSO2 4通道熒光光纖氧氣測(cè)量?jī)x，對(duì)Aquitaine海岸沉積樣芯耗氧進(jìn)行了測(cè)量分析，以研究海洋底棲動(dòng)物活動(dòng)（bioirrigation）對(duì)海岸帶生態(tài)系統(tǒng)生態(tài)過(guò)程及生物地理化學(xué)功能（如沉積有機(jī)物的再礦化）的影響。

案例2：芬蘭Turku大學(xué)利用FSO2和430μm光極氧探針，對(duì)南瓜類囊體懸浮液光合放氧進(jìn)行了測(cè)量分析。

案例3：美國(guó)Woods Hole海洋學(xué)研究所，利用RF-O2非接觸式光極氧氣傳感器（sensor spot），對(duì)海洋無(wú)脊椎動(dòng)物呼吸代謝進(jìn)行了測(cè)量分析，以研究其固有的生物鐘與環(huán)境脅迫的關(guān)系，這些海洋無(wú)脊椎動(dòng)物體重只有0.5－50mg。圖中為翼足類軟體動(dòng)物在不同濃度CO2條件下的耗氧率。

案例4：澳大利亞海洋科學(xué)研究所、瑞典Gothenburg大學(xué)等組成的科學(xué)小組，利用Pyroscience的REDFLASH氧氣測(cè)量技術(shù)，對(duì)河鱸（Perca fluviatilis）呼吸代謝進(jìn)行測(cè)量分析，以研究其熱耐受性和適應(yīng)性的生理機(jī)制。他們選擇波羅的海核電站附近的一個(gè)瀉湖，核電站排出的熱水進(jìn)入該瀉湖，在過(guò)去30年大量魚類因?yàn)椴贿m應(yīng)水溫升高而滅絕，但河鱸卻得以繁盛，該地成為理想的研究氣候變暖對(duì)魚類種群影響的“天然實(shí)驗(yàn)室”。他們測(cè)量河鱸呼吸代謝率的同時(shí)，還測(cè)量其靜脈血液在溫度升高狀態(tài)下的氧分壓，靜脈血是河鱸心臟供氧的主要來(lái)源，高溫條件下靜脈血氧氣含量被認(rèn)為是其心臟功能的重要限制因子。

案例5：德國(guó)Ulm大學(xué)利用FSO2測(cè)量?jī)x和50μm可伸縮式RFO2探針，對(duì)患者腦脊髓液（CSF）樣品溶解氧進(jìn)行測(cè)量分析，以研究探討神經(jīng)紊亂及神經(jīng)炎等疾病的生理和診斷。

案例6：德國(guó)農(nóng)業(yè)科學(xué)與景觀研究機(jī)構(gòu)，利用FSO2測(cè)量?jī)x和RFO2探針，對(duì)土壤氧氣進(jìn)行測(cè)量，以評(píng)估不同種類蚯蚓在低氧條件下對(duì)土壤改良的效率。

案例7：西班牙Valladolid大學(xué)利用RFO2熒光光纖氧氣測(cè)量技術(shù)，監(jiān)測(cè)葡萄酒橡木桶O2吸收——對(duì)葡萄酒品質(zhì)至關(guān)重要但一直以來(lái)缺乏科學(xué)的了解。葡萄酒在橡木桶內(nèi)（3－24個(gè)月）的過(guò)程溶解氧至關(guān)重要，因?yàn)镺2調(diào)節(jié)了葡萄酒整個(gè)的熟化過(guò)程。

近期部分參考文獻(xiàn)：

2015

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2014

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25. Increased rates of dissimilatory nitrate reduction to ammonium (DNRA) under oxic conditions in a periodically hypoxic estuaryRoberts et al., 2014, Geochim Cosmochim Acta Vol 133: 313-324

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